Cell Suspension Culture of Papaver Nudicaule and Its Alkaloids Producti on

Cell suspension cultures of Papaver nudicaule were o btained and their alkaloids content was determined by HPLC. The biomass of suspension cells and th e yield of alkaloids can be accelerated after the supplement of nutritions in th e middle or late phase of the growth cycle. It has been showed that 3% sucrose w as most favorable to the cell growth and 4% sucrose to the yield of total alkalo ids by the study of the carbone source. The research of the original pH value of medium showed that the biomass of suspension cells got the fastest growth at pH 5.7 in the medium, and the highest yield of total alkaloid at pH 5.4. The amoun t of nudicauline in the cell by suspension culture was 0.0235 mg/g DW and the a muresinine was 0.022mg/g DW respectively by means of HPLC.