Fuel of proton exchange membrane fuel cell(PEMFC) mostly comes from reformate contains CO which will poison the fuel cell electrocatalyst.The effect of CO on the performance of PEMFC was studied in this paper.The results of the experiment show that when CO is fed in PEMFC,the cell voltage will be decreased violently,then it will become steady with some vibration.The poison will become more serious with higher CO content and weaker at higher temperature.The adsorption of CO on the electrocatalyst is reversible. The PtRu electrocatalyst is more suitable for the electrocatalysis of CO/H 2 than Pt electrocatalyst.