Experimental Study about Total Alkaloids from Rubus alceaefolius Poir on Treatment of Nonalcoholic Fatty Liver Rat

Objective To study the role and mechanism of total alkaloids from Rubus alceaefolius Poir(TARAP) for the treatment of nonalcoholic fatty liver(NFL).Methods 60 SD rats were randomly divided into blank control group,model group,polyene phosphatidylcholine(PPC) group and TARAP groups with different doses respectively.The rats were fed with high fat forage for 12 weeks to establish the model of NFL.After successive administration for 4 weeks,the content changes of crude fat of hepatic tissue and serum TG,TC,LDL-C and HDL-C were observed,and the pathological change of hepatic tissue was observed by HE stain.Results The content of crude fat of hepatic tissue and concentrations of serum TG and TC were decreased in TARAP groups with different doses respectively,and the decreasing amplitude was higher than that of control group respectively(P0.05).The concentration of serum HDL-C was increased and LDL-C was decreased in middle and high dose group of TARAP respectively,and the increasing and decreasing amplitude was higher respectively than that of control group(P0.05).HE stain showed both PPC and TARAP could lighten the steatosis of liver cells,reduce lipid aggregation in hepatic tissue,and there was a dose-effect relationship between them.Conclusion TARAP has significant effect on NFL rats,and its mechanism is probably related with the effect of regulating disorder of lipid metabolism.