Determination of Polyunsaturated Fatty Acids in Seal Oil by GC/MS

To establish a GC/MS method for the determination of polyunsaturated fatty acids in seal oil. The sample was prepared from seal oil, then the components of fatty acid in it were determined by GC/MS. Their relative contents were measured by normalized method of chromatographic peak areas. 25 kinds of fatty acids were separated from seal oil, of which the main components, Eicosapentaenoic acid(EPA), Docosahexaenoic acid(DHA) and Docosapentaenoic acid(DPA),were analysized qualitatively. The results were the same as the related literatures reported. [CONCLUSION]This method is simple, sensitive and rapid.