The infusing rules of effective materials in green tea brewed with room temperature water was studied in the paper. The possibility and necessary of brewing green tea with room temperature water was also studied by contrasting the infusing rules under the different brewing temperature. The results showed that the rules of infusing with different brewing temperature were similar. The infusing velocity of dissoluble materials in green tea brewed with room temperature was slower than those brewed with higher temperature, so does the time of reaching the saturation concentration. Brew the tea with room temperature water for 2h, the tea was still heavy and mellow, without stewed taste. The paper also showed that the best time of green tea brewed with room temperature water was 40min.