Influence of angiotensin-(1-7) on expression of E-cadherin andα-SMA induced by angiotensinIIin rats tubular epithelial cells

Objective To investigate the influence of angiotensin-(1-7)[Ang-(l-7)]on expression of E-cadherin and a-SMA induced by angiotensionⅡ(AngⅡ) in rat's tubular epithelial cells.Methods The NRK52E were maintained and sub cultured,and then treated with Ang-(1-7)(10~(-6)mol/L) and AngⅡ(10~(-6)mol/L) for 24,48,72,96 hours,the protein expressions of E-cadherin andα-SMA were detect by immunocytochemistry method;The mRNA expression of E-cadherin and a-SMA was detected by real time PCR(RT-PCR).Results Treated with angⅡafter 96 h,the protein and mRNA expression of E-cadherin decreased significantly(P0.05 ),but the protein and mRNA expression ofα-SMA increased significantly (P0.05);treated with AngⅡand Ang-(1-7),the protein and mRNA expression of E-cadherin increased significantly (P0.05),but the protein and mRNA expression ofα-SMA decreased significantly(P0.05).Conclusion Ang-(1-7) can inhibit the expression ofα-SMA induced by AngⅡand up regulate the expression of E-cadherin.