Determination of four components in Tangmaikang granule by high-performance liquid chromatography

OBJECTIVE To control Tangmaikang granule quality according to their contents of astragaloside Ⅳ,7-hydroxy-4'-methoxyisoflavone,7,3'-dihydroxy-4'-methoxy-isoflavone,paeoniflorin.METHODS HPLC-ELSD was used to determine astragaloside Ⅳ.RP-HPLC was used to determine 7-hydroxy-4'-methoxyisoflavone,7,3'-dihydroxy-4'methoxy-isoflavone and paeoniflorin. RESULTS Linear range and correlation coefficients of paeoniflorin, 7-hydroxy-4'-methoxyisoflavone,7,3'-dihydroxy-4'-methoxy-isoflavone and astragaloside Ⅳ were 1.85×10-2~9.25×10-1μg,r=0.999 5;4.700×10-3~2.820×10-2μg,r=0.999 5;4.650×10-3~2.790×10-2μg,r=0.999 8;4.032×10-2～0.282μg,r=0.999 4 respectively.CONCLUSION As a whole,the contents of four component were unvariable in Tangmaikang granule.The method that according to their content of multi-component control quality of Tangmaikang granule was practical.