RNA聚合酶Ⅱ
延伸系数
抄写(语言学)
分子生物学
生物
细胞生物学
聚合酶
RNA聚合酶Ⅲ
核糖核酸
化学
RNA聚合酶
DNA
发起人
基因
基因表达
遗传学
核糖体
哲学
语言学
作者
Seychelle M. Vos,Lucas Farnung,Henning Urlaub,Patrick Cramer
出处
期刊:Nature
[Springer Nature]
日期:2018-08-01
卷期号:560 (7720): 601-606
被引量:265
标识
DOI:10.1038/s41586-018-0442-2
摘要
Metazoan gene regulation often involves the pausing of RNA polymerase II (Pol II) in the promoter-proximal region. Paused Pol II is stabilized by the protein complexes DRB sensitivity-inducing factor (DSIF) and negative elongation factor (NELF). Here we report the cryo-electron microscopy structure of a paused transcription elongation complex containing Sus scrofa Pol II and Homo sapiens DSIF and NELF at 3.2 Å resolution. The structure reveals a tilted DNA–RNA hybrid that impairs binding of the nucleoside triphosphate substrate. NELF binds the polymerase funnel, bridges two mobile polymerase modules, and contacts the trigger loop, thereby restraining Pol II mobility that is required for pause release. NELF prevents binding of the anti-pausing transcription elongation factor IIS (TFIIS). Additionally, NELF possesses two flexible ‘tentacles’ that can contact DSIF and exiting RNA. These results define the paused state of Pol II and provide the molecular basis for understanding the function of NELF during promoter-proximal gene regulation. The cryo-electron microscopy structure of a paused transcription elongation complex of RNA polymerase II bound to DRB sensitivity-inducing factor and negative elongation factor is reported at 3.2 Å resolution.
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