BRONCHIAL MICROBIOME IN PATIENTS WITH LUNG CANCER

Background: The local inflamatory environment of a tumour, and its evolution, can be conditioned by the surrounding microbiome. The propose of this study was to compare the lung microbiome of affected bronchus with the contralateral health bronchus, oral and the intestinal microbiome Patients and Methods: 19 patients (18 males, mean age of 72 years) diagnosed of squamous (7), adenocarcinoma (6) and small cell (4) lung cancer were included. Bronquial biopsy was obtained by broncoscopy from tumoral affected bronchus and contralateral healthy bronchus. Each patient also provided a faecal sample and an oral rinse. Total DNA was obtained using the QiaAmp kit (Qiagen), and microbiome composition was determined by massive sequencing in MiSeq platform (Illumina) Results: No significant differences were detected between the microbiome from the affected bronchus and contralateral healthy bronchus. The dominant genera were Blautia, Streptococcus and Prevotella among a total of 280 bacterial species. Oral microbiome composition was uniform, whereas considerable differences were detected in the gut compartment Conclusions: Bronchial microbiome of patients with lung cancer seems to be quite uniform, without significant differences between the tumoral and non-tumoral involved tissues. As it can be expected, bronchial microbiome has important similarities with the oral one, but with some own features. Comparing this bronchial microbiome with the one from non-lung cancer, could be subject of interest