辣椒素
爪蟾
烟碱激动剂
乙酰胆碱
抑制性突触后电位
化学
电压钳
生物物理学
乙酰胆碱受体
兴奋剂
膜片钳
受体
烟碱乙酰胆碱受体
细胞生物学
生物
药理学
内分泌学
膜电位
生物化学
基因
作者
Asma Hassan Alzaabi,Luke Howarth,Eslam El Nebrisi,Nurulain Syed,Kun Yang,Frank Christopher Howarth,Murat Öz
标识
DOI:10.1016/j.ejphar.2019.172411
摘要
Capsaicin is a naturally occurring alkaloid derived from Chili peppers fruits. Using the two-electrode voltage-clamp technique in Xenopus oocyte expression system, actions of capsaicin on the functional properties of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor were investigated. Ion currents activated by ACh (100 μM) were reversibly inhibited with an IC50 value of 8.6 μM. Inhibitory actions of capsaicin was independent of membrane potential. Furthermore, Ca2+-dependent Cl- channels expressed endogenously in oocytes were not involved in inhibitory actions of capsaicin. In addition, increasing the ACh concentrations could not reverse the inhibitory effects of capsaicin. Importantly, specific binding of [125I] α-bungarotoxin remained unaltered by capsaicin suggesting that its effect is noncompetitive. Whole cell patch-clamp technique was performed in CA1 stratum radiatum interneurons of rat hippocampal slices. Ion currents induced by choline, a selective-agonist of α7-receptor, were reversibly inhibited by 10 min bath application of capsaicin (10 μM). Collectively, results of our investigation indicate that the function of the α7-nACh receptor expressed in Xenopus oocytes and in hippocampal interneurons are inhibited by capsaicin.
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