Two-photon fluorescence imaging of mitochondrial superoxide anion transport mediating liver ischemia-reperfusion injury in mice

We constructed a two-photon fluorescence ratio probe (CST) for in situ quantitative real-time detection of mitochondrial O₂˙^-. Fluorescence imaging showed that O₂˙^- was over-generated from mitochondria and conveyed to the cytoplasm via voltage-dependent anion channels in hepatic ischemia-reperfusion mice, damaging the important functional protein aconitase in the cytoplasm.