微泡
癌症干细胞
癌症研究
转移
外体
生物
上皮-间质转换
间质细胞
小RNA
间充质干细胞
干细胞
肾透明细胞癌
肺癌
细胞
下调和上调
细胞周期
细胞生物学
癌症
肿瘤微环境
细胞生长
病理
肾细胞癌
医学
基因
生物化学
遗传学
作者
Lu Wang,Guang Yang,Danfeng Zhao,Jiaqi Wang,Yang Bai,Qiang Peng,Hongzhi Wang,Ruizhe Fang,Guang Chen,Zhichao Wang,Keliang Wang,Guangbin Li,Yinhui Yang,Ziqi Wang,Guo Pengyu,Li Peng,Dayong Hou,Wanhai Xu
标识
DOI:10.1186/s12943-019-0997-z
摘要
Abstract Background Clear cell renal cell carcinoma (CCRCC) is characterized by a highly metastatic potential. The stromal communication between stem cells and cancer cells critically influences metastatic dissemination of cancer cells. Methods The effect of exosomes isolated from cancer stem cells (CSCs) of CCRCC patients on the progress of epithelial-mesenchymal transition (EMT) and lung metastasis of CCRCC cells were examined. Results CSCs exosomes promoted proliferation of CCRCC cells and accelerated the progress of EMT. Bioactive miR-19b-3p transmitted to cancer cells by CSC exosomes induced EMT via repressing the expression of PTEN. CSCs exosomes derived from CCRCC patients with lung metastasis produced the strongest promoting effect on EMT. Notably, CD103 + CSC exosomes were enriched in tumor cells and in lung as well, highlighting the organotropism conferred by CD103. In addition, CD103 + exosomes were increased in blood samples from CCRCC patients with lung metastasis. Conclusions CSC exosomes transported miR-19b-3p into CCRCC cells and initiated EMT promoting metastasis. CD103 + acted to guide CSC exosomes to target cancer cells and organs, conferring the higher metastatic capacity of CCRCC to lungs, suggesting CD103 + exosomes as a potential metastatic diagnostic biomarker. Graphical abstract ᅟ
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