生物
受体
生物化学
激酶
蛋白激酶A
蛋白激酶结构域
细胞生物学
分子生物学
基因
突变体
作者
Yoshikatsu Matsubayashi,Mari Ogawa,Akiko Morita,Youji Sakagami
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2002-05-24
卷期号:296 (5572): 1470-1472
被引量:350
标识
DOI:10.1126/science.1069607
摘要
The sulfated peptide phytosulfokine (PSK) is an intercellular signal that plays a key role in cellular dedifferentiation and proliferation in plants. Using ligand-based affinity chromatography, we purified a 120-kilodalton membrane protein, specifically interacting with PSK, from carrot microsomal fractions. The corresponding complementary DNA encodes a 1021–amino acid receptor kinase that contains extracellular leucine-rich repeats, a single transmembrane domain, and a cytoplasmic kinase domain. Overexpression of this receptor kinase in carrot cells caused enhanced callus growth in response to PSK and a substantial increase in the number of tritium-labeled PSK binding sites, suggesting that PSK and this receptor kinase act as a ligand-receptor pair.
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