生物
大肠杆菌
核糖体结合位点
基因
核糖体
遗传学
基因表达
核糖核酸
结合位点
分子生物学
作者
Peter O. Olins,Catherine S. Devine,Shaukat H. Rangwala,Kamilla S. Kavka
出处
期刊:Gene
[Elsevier]
日期:1988-12-01
卷期号:73 (1): 227-235
被引量:176
标识
DOI:10.1016/0378-1119(88)90329-0
摘要
Expression of foreign genes in Escherichia coli requires the juxtaposition of prokaryotic transcription and translation elements with a coding region for the foreign gene. Commonly, this results m only modest expression of the foreign gene product. Here we describe a novel ribosome-binding site (RBS; phage T7 ‘gene 10 leader’) which is able to drive the translation of several foreign genes. This RBS dramatically enhanced the translation efficiency of all the genes we have tested to date, and was particularly effective for foreign genes. The enhanced expression was often more than 40-fold greater than that obtained using a ‘consensus’ RBS. A general plasmid vector has been constructed, incorporating the T7 gene 10 leader sequence, which allows the facile expression of important gene products. In this report we demonstrate the application of this system for the high-level expression of plant, mammalian and bacterial proteins in E. coli.
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