药品
芯片上器官
细胞培养
器官系统
串扰
器官培养
病理
体外
药理学
人体皮肤
生物医学工程
生物
材料科学
医学
纳米技术
生物化学
微流控
工程类
疾病
遗传学
电子工程
作者
Ilka Wagner,Eva-Maria Materne,Sven Brincker,Ute Süßbier,Caroline Frädrich,Mathias Busek,Frank Sonntag,D. A. Sakharov,Evgeny V. Trushkin,A. G. Tonevitsky,Roland Lauster,Uwe Marx
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2013-01-01
卷期号:13 (18): 3538-3538
被引量:392
摘要
Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, to accurately predict drug toxicity. In this study, we present a multi-organ-chip capable of maintaining 3D tissues derived from cell lines, primary cells and biopsies of various human organs. We designed a multi-organ-chip with co-cultures of human artificial liver microtissues and skin biopsies, each a 1/100 000 of the biomass of their original human organ counterparts, and have successfully proven its long-term performance. The system supports two different culture modes: i) tissue exposed to the fluid flow, or ii) tissue shielded from the underlying fluid flow by standard Transwell® cultures. Crosstalk between the two tissues was observed in 14-day co-cultures exposed to fluid flow. Applying the same culture mode, liver microtissues showed sensitivity at different molecular levels to the toxic substance troglitazone during a 6-day exposure. Finally, an astonishingly stable long-term performance of the Transwell®-based co-cultures could be observed over a 28-day period. This mode facilitates exposure of skin at the air–liquid interface. Thus, we provide here a potential new tool for systemic substance testing.
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