Andrographolide from andrographis paniculata extract: optimization of accelerated solvent extraction technique

Andrographolide is main diterpenoid lactone isolated from Andrographis paniculata present mostly in the leaves and all part of the plant. It is widely use as pharmaceutical and nutraceutical formulation. In this study, andrographolide from Andrographis paniculata were extracted applying technique of accelerated solvent extraction (ASE). The extracts obtained were characterized by HPLC. The quantification method showed good linearity (r2= 0.9107) and precision (RSD < 5%) with low values of detection (LOD) and quantification (LOQ) limits. A Box–Behnken design was used to correlate three independent variables (temperature, extraction time and number of cycles) with the amount of andrographolide extracted. The response surface methodology was applied to optimize the extraction of andrographolide by ASE. The optimal conditions were 80oC, 5 minutes and 2 cycle. The shorter time of extraction and the lower heat energy used justify the ASE technique choice to characterize andrographolide from Andrographis paniculata.