Effects of unification medication of glycyrrhizinate and 5‐ASA on expression of TNF‐α,IL‐8 and NF‐κB in rats model with ulcerative colitis

Objective To explore mechanism of molecule of glycyrrhizinate and 5‐aminosalicylic acid(5‐ASA) treating ulcerative colitis（UC） through observing expression of TNF‐α,IL‐8 and NF‐κB in rats with UC induced by 2,4‐dinitro‐chlorobenzene and acetic acid. Method 50 male Wistar rats were randomly divided into 5 groups: normal control group, UC model group,5‐ASA group, glycyrrhizinate group and unification medication group.(10 rats in each group). The rats in normal control group and UC model group were given normal saline, the rats in 5‐ASA group were given 5‐ASA (1ml/200g weight), the rats in glycyrrhizinate group were given glycyrrhizinate(1ml/200g weight), the rats in unification medication group were given 5‐ASA(1ml/200g weight) and glycyrrhizinate(1ml/200g weight). Symptom of bowel complaint and hemafecia in rats was observed and the change of body weight was recorded every day.After 7 days, blood of rats were got, the level of TNF‐α、IL‐8 in the blood serum was evaluated by ELISA. Colons(8cm) of rats were got, score of colonic damage was determined, the level of NF‐κB in colonic mucosa was determined by immunohistochemistry． Result In the stage of UC, score of colonic damage advanced obviously, the level of TNF‐α，IL‐8 in the blood serum and expression of NF‐κB in colonic mucosa advanced obviously, and those were correlated with change of state of the illness; After using glycyrrhizinate or 5‐ASA, score of colonic damage in every group degraded obviously, the level of TNF‐α，IL‐8 in the blood serum and expression of NF‐κB in colonic mucosa descended obviously; The effects of unification medication of glycyrrhizinate and 5‐ASA lowering the level of TNF‐α，IL‐8 in the blood serum and expression of NF‐κB in colonic mucosa were more excellent than that of single sentero clyster by using 5‐aminosalicylic acid or glycyrrhizinate ( P <0.05) . Conclusion Unification utilizing glycyrrhizinate and 5‐ASA had a good therapeutic action for rats with ulcerative colitis evoked by 2,4‐Dinitro‐chlorobenzene and acetic acid. Its mechanism of action might be to reduce damage of inflammation by suppressing of expression of TNF‐α，IL‐8 and NF‐κB in rats .