清脆的
干细胞
基因
生物
基因敲除
基因表达
细胞生物学
富含亮氨酸重复
计算生物学
遗传学
作者
Swetlana Rot,Matthias Kappler
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 255-268
标识
DOI:10.1007/978-1-0716-1225-5_18
摘要
The modulation of gene expression is essential for the investigation of function or involved pathway of a single gene of interest, in particular in the developmental/stem cell biology. The temporary knock down of gene expression via siRNA is a well-established but with a residual expression connected modulation method. The chapter describes the complete knockout of a defined target and allows a comprehensive study of different gene like the stem cell gene LGR4 (Leucine-rich repeat-containing G-protein-coupled receptor 4) using the new developed CRISPR/Cas method (clustered regularly interspaced short palindromic repeats).
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