作者
Qixiang Zhang,Wenbin Chen,Lidan Sun,Fang Zhao,Bangqing Huang,Weiru Yang,Ye Tao,Jia Wang,Yuan Zhou,Guangyi Fan,Zhen Xing,Changlei Han,Huitang Pan,Xue Zhong,Wenfang Shi,Xinming Liang,Dongliang Du,Fengming Sun,Zongda Xu,Ruijie Hao,Tian Lv,Yingmin Lv,Zequn Zheng,Ming Sun,Le Luo,Ming Cai,Yu Gao,Junyi Wang,Ye Yin,Xun Xu,Tangren Cheng,Jun Wang
摘要
Prunus mume (mei), which was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae been sequenced. Here, we assemble a 280M genome by combining 101-fold next-generation sequencing and optical mapping data. We further anchor 83.9% of scaffolds to eight chromosomes with genetic map constructed by restriction-site-associated DNA sequencing. Combining P. mume genome with available data, we succeed in reconstructing nine ancestral chromosomes of Rosaceae family, as well as depicting chromosome fusion, fission and duplication history in three major subfamilies. We sequence the transcriptome of various tissues and perform genome-wide analysis to reveal the characteristics of P. mume, including its regulation of early blooming in endodormancy, immune response against bacterial infection and biosynthesis of flower scent. The P. mume genome sequence adds to our understanding of Rosaceae evolution and provides important data for improvement of fruit trees. The Prunus mume was domesticated in China over 3,000 years ago and is an important ornamental plant and fruit. Here Qixiang Zhang et al.obtain the first assembly of its genome with a combination of next-generation sequencing, whole-genome mapping and restriction-site-associated DNA.