光遗传学
转化生长因子
信号转导
转化生长因子β
细胞信号
生物
转化生长因子β信号通路
细胞生物学
神经科学
作者
Yuchao Li,Minji Lee,Nury Kim,Guoyu Wu,Difan Deng,Jin Man Kim,Xuedong Liu,Won Do Heo,Zhike Zi
标识
DOI:10.1021/acssynbio.7b00225
摘要
Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-β signaling in time and space. Using the optoTGFBRs system, we show that TGF-β signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-β receptor and Smad2 proteins, we characterized the dynamics of TGF-β signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-β signaling at the single cell level.
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