柠檬酸
糖尿病肾病
化学
分析物
色谱法
肾病
糖尿病
定量分析(化学)
生物标志物
接收机工作特性
肌酐
内科学
内分泌学
生物化学
医学
作者
Haoxue Gao,Xiaoyi Yu,Runbin Sun,Na Yang,Jun He,Mingxue Tao,Huilin Gu,Caixia Yan,Jiye Aa,Guangji Wang
标识
DOI:10.1016/j.jchromb.2017.12.021
摘要
The early diagnosis of diabetic nephropathy (DN) is rather challenging. Our previous study suggested that citric acid is a potential marker for the early diagnosis of diabetic nephropathy in db/db mice. For the first time, in this study, a surrogate analyte of 13C6-citric acid was employed to generate calibration curves for the quantitative measurement of the endogenous citric acid in the sera of db/db mice and diabetic nephropathy patients by GC/MS after the analytes were extracted, methoximated and trimethylsilylated. The constant response factor of 13C6-citric acid versus citric acid over the linear range indicated the identical ionization efficiency of these two compounds. The full validation assessments suggested that the method is sensitive, specific, reliable, reproducible and has acceptable parameters. Statistical analysis revealed cut-off citric acid concentrations of 29.24 μg/mL with a 95% confidence interval between 32.75 and 39.16 μg/mL in the diabetic nephropathy patients and 16.74 and 22.57 μg/mL in the normal controls. The areas under the receiver operating characteristic curves indicated accuracies of over 90% for the diagnoses of early diabetic nephropathy in both humans and db/db mice, which suggests that the serum citric acid level is potentially a biomarker that could assist in the diagnosis of diabetic nephropathy.
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