Research on Dihuang Yinzi Decoction’s neuroprotection effects on MCAO rats from the HPA axis reaction and apoptosis aspects

Objective To explore the Dihuang Yinzi Decoction’s neuroprotection effects on MCAO rats from the HPA axis reaction and apoptosis aspects. Methods 30 SD rats were randomly divided into 3 groups: MCAO model group,herb formula group,and normal group,each group was 10. For drug treatment,herb formula group were given Dihuang Yinzi Decoction from 2 days to 8 days after ischemia by intragastric administration method,whereas the MCAO model group and normal group received equivalent volumes of distilled water. SD rats were killed 1h after the last intragastric administration. The expression levels of ACTH,CRH,COR were detected by radioimmunoassay. The apoptosis of neuron was detected by TUNEL Immunohistochemistry. Results The level of COR in herb formula group were significantly lower than that in normal group and MCAO model group( P 0. 01) ; The level of ACTH,CRH was significantly higher in herb formula group than in normal and MCAO model group( P 0. 05) ; The expression of apoptosis in neuron was significantly lower in herb formula group than in MCAO model group ( P 0. 01) . Conclusion Dihuang Yinzi Decoction may exert neuroprotection mechanism and improve the prognosis through restoring the disturbed condition of HPA axis reaction and inhibiting apoptosis.