The impact effect of ecology caused by agricultural non- point source pollution may be called all-directional.The most direct and notable harm caused by agricultural non- point source pollution is pollution of surface water . It is two fallowing aspects in the pollution of the environment of water body: the pollution of nutrition type and poison type . In order to find out the pollution situation in surface water of agricultural non- point source pollution of Zhu Xi River region, this paper analyze the content of samples in lead , mercury , phosphor, nitrogen , COD and volatilizable phenol . So let us recognizing the harm of agricultural non- point source pollution, discussing the control method.