Discussion on the pathogenic microorganism cultivation method from feces samples of diarrhea patients

Objective To explore the enteropathogen cultivation method from feces samples of diarrhea patients.Methods Feces samples were tested by microorganism inspectors with routine observation and gram stain or acid-fast stain microscopic examination,and then blood agar plate with 5%,Chinese orchid agar plate,SS agar plate,peptone alkaline water media were used for general bacteria cultivation.Results We retrospective analyzed the feces samples of pathogenic microorganism from 200 diarrhea patients,and found out that the salmonella bacteria and bacterial isolated by traditional method were not the main enteropathogens.Conclusion The traditional and single method of SS agar plate is difficult to adapt to the changes of enteropathogens.Using feces samples from diarrhea patients cultured by blood agar plate with 5%,Chinese orchid agar plate,SS agar plate and peptone alkaline water media after routine observation and gram stain or acid-fast stain microscopic examination by microorganism inspectors could improve the reorganization rate of pathogenic bacteria.