EFFECTS OF CULTURE CONDITIONS ON THE ALKALOID YIELD OF Fritllarla ussurensis MAXIM FROM BULB in vitro

Factors(including culture media, culture methods, time, plant growth regulators, sucrose concentration in the medium as well as ribavirin) influencing the alkaloid yield of Fritillaria ussurensis Maxim from bulb in vitro were investigated by evaluation of the bulb growth, the alkaloid content, the ratio of dry weight and fresh weight (DW/FW), the alkaloid yield, etc. The result showed that LS solid medium was better than others on the yield of the alkaloid. The highest yield of 1.62 mg/L was achieved from LS solid medium with 1.0 mg/L BA, 0.5 mg/L NAA, 5mg/L ribavirin as well as 5mg/L sucrose after culture 60 d. The yield and the content of alkaloid had no significant increase on the LS liquid medium with the same condition as the solid one, however, liquid culture stimulated the bulb propagation.