Immunophenotyping of 185 Children with Acute Leukemia by Flow Cytometry

Objective:To investigate the significance of immunophenotype in children's acute leukemia via flow cytometry. Methods:Immunophenotypes were detected by three-color flow cytometry(FCM) and CD45/SSC gating in 185 cases of acute leukemia (AL) and antigentic expressions were analysed.Results:The coincidence rate was 89.19%between FAB and immunophenotype.Among the 185 cases of AL,121(65.41%) were acute lymphoblastic leukemia(ALL).In 113 cases of B-lineage ALL(B-ALL),CD19(99.12%), CD22(98.13%),CD79a(96.19%),CD10(86.73%)agents had high expression.CD 19 was the most sensitive and CD79a was the most intense in specificity.In 8 cases of T-ALL,CD5(100%),CD7(100%),cCD3(100%),CD8(87.5%)agents had higher expression in T lymphoblastic,CD5 and CD7 were the most sensitive,cCD3 was the most intense in specificity.In AML,CD33 was high sensitivity and MPO was specificity.Myeloblastic agents were often expressed as follows:CD33,CD15,CD64,MPO,CD13 in the order of decreasing.17 cases were acute mixed lineage leukemia,9 cases were B/M,5 cases were T/B and 3 case were T/M.My+-ALL made up 44.63%of ALL express such myeloid antigens as CD13,CD15,CD33,CD64.18 cases were Ly+-AML,in which CD19、CD4 and CD7 were chiefly presented Lymphoid lineage associated antigents.The unlineage-associated antigens expression frequencies were as follows: CD34(67.57%),HLA-DR(85.41%),CD38(80.59%),TdT(62.59%).Conclusion:Immunophenotype plays an important role in the classification of children's acute leukemia and is a supplementary and correction of FAB phenotype.It can enhance the exactness of diagnosis and needs to be standardized.