The Culture of Tissue Cells in Clots Formed from Purified Bovine Fibrinogen and Thrombin.

SummaryProcedures are described for the successful culture of tissue cells in clots formed from purified bovine fibrinogen and thrombin. The influence on clot characteristics of pH and temperature, effective during clot formation, and the influence of fibrinogen concentration on the subsequent cell migration in the clot are indicated. Cultures grown in these clots can be transferred with the same ease as those grown in clots derived from chick plasma. The problem of fibrinoly-sis is briefly mentioned.