Study on Protein Extraction Methods of Pomegranate Leaves

Taking the leaves of Punicagranatum Linn ‘Dahongpao’as material,three protocols for total protein extraction,trichloroacetic acid/acetone precipitation(TCA),phe-nol extraction methanol/ammonium acetate precipitation and Tris-HCl extraction were compared.The results showed that TCA/Acetone extration was unclear background;the Tris-HCl extraction was the most appropriate for pomegranate leaf proteome analysis on account of the highest resolution and more informative.