Advance in Cell Electrofusion Technology

As one of the most widely used technology in cell fusion, cell electrofusion is a rapidly developing cell engineering method, which utilizes dielectrophoretic force to induce cell alignment. Aligned cells are electroporated and subsequently electrofused within high electric field strength. These fused cells obtain different genetic materials from individual parent cells, which can show new genetic characteristics. At present, cell electrofusion is of important significance in biomedical and agricultural research. In the paper, the theoretical fundamental of electrofusion and up-to-date development are reviewed. Some relative research in our laboratory is also described.