高分辨率熔体
放大器
桑格测序
熔化曲线分析
分子生物学
DNA测序
生物
突变
序列(生物学)
序列分析
DNA
基因
遗传学
计算生物学
聚合酶链反应
作者
Meenakshi Mehrotra,Keyur P. Patel
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 63-69
被引量:1
标识
DOI:10.1007/978-1-4939-3360-0_7
摘要
High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
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