The Effect of Spleen-reinforcing Therapy on the Transporting and Distribution of 59 Fe Metablism of Spleen-deficiency-suffering Mice in the Organs

Objective:The spleen-deficiency-suffering mice models are observed dynamically on the perspective of pharmacokinetics and by radioactive tracer method,and the effect of spleen-reinforcing therapy on the mice's obsorption of Fe and the process of transporting and distribution is observed as well,so that the relationship between the spleen's function of dominating transporting/ dysfunction of the spleen in transporting and Fe metabolim is researched; The biological meaning of spleen's function of dominating transporting is discussed;The scientific content of the important TCM theories of spleen's dominating the transporting and spleen's determining the condition of the acquired construction is revealed;the experimental basis is provided for the research on spleen's functions and /or the essence of spleen-deficiency syndrome.Method: 80 Male SD mice are divided by random into normal group,normal reinforcing-spleen group,spleen-deficiency group,reinforcing-spleen-deficiency group.The mice model are fed 59 Fe Cl3 and 0.2 ml water-floating fluid at one time;the mice of the groups of normal reinforcing-spleen and reinforcing-spleen-deficiency are fed 1.5ml 1:1 Si Jun Zi Tang;the groups of normal and spleen-deficiency are fed 1.5 ml normal sanile once a day.3 rats of each group will be selected by random at 0.5,1,2,4,8 and 14 phase and are made dead by eyeball blood letting method,and then radio 59 Fe level is measured by Y radio-immunity counter after the organs and tissues of spleen,liver,small intestine,stomach and kidney are extracted,and the pharmacokinetics change of radio Fe's transporting and distribution from the whole body's circulatory system to the rats's tissues and organs.Results: 59 Fe transporting exists significant difference in the condition of spleen-deficiency and reinforcing-spleen,the therapy of reinforcing-spleen improve the transporting and distribution of 59 Fe of the mice.Conclusions: 59 Fe's transporting process from blood circulatory system to the organs and tissues decreased in the condition of spleen-deficiency,the therapy of reinforcing-spleen can promote the transporting of 59 Fe in the tissues and organs of the mice,especially concentrating on the spleen,which shows that Si Jun Zi Tang has the function of regulating the spleen-deficiency by reinforcing the spleen so that the substance transporting system of the organism is regulated.