Effect of Infliximab on TNF-α of guinea pigs asthma model

Objective To investigate the effect of Infliximab on TNF-α of guinea pigs asthma model.Methods Guinea pigs(50),half male and female,were randomly divided into five groups,control,model,low-,mid-,and high-dose Infliximab(5,10,and 15 mg/kg)groups(n=10).Model and the Infliximab groups were ip administered with 10% ovalbumin to sensitivate,and the control group was given physiological saline.Model and Infliximab groups were atomizing inhalated twice with 2% ovalbumin solution,and the control group was given the same dose physiological saline.Infliximab groups were ip administered with appropriate doses of Infliximab before excitation each time,and animals were killed 24 h after the second excitation.TNF-α was detected in serum,tissue,and bronchoalveolar lavage fluid.Results TNF-α concentration in the serum,tissue homogenates,and bronchoalveolar lavage fluid in the model group was higher than that in the control group(P 0.05).However,TNF-α concentration in the serum,tissue homogenates,and bronchoalveolar lavage fluid of the Infliximab group was lower than that in the model group(P 0.05).Conclusion TNF-α is related to the formation of the guinea pig asthma,and Infliximab could reduce TNF-α of serum,tissue homogenates,and bronchoalveolar lavage fluid in guinea pig model of asthma.