Expression and resistance of plasmid-mediated AmpC gene in continuous isolates from Escherichia coli and Klebsiella pneumoniae

Objective To study the expression and resistance of plasmid-mediated AmpC gene in continuous isolates from Escherichia coli and Klebsiella pneumoniae. Methods The DHA and ATC-1 types of AmpC enzymes in continuous isolates from Escherichia coli and Klebsiella pneumoniae were detected by polymerase chain reaction(PCR). Results In 68 isolates Escherichia coli, there are 4 isolates, whose ATC-1 types were positive, and the DHA types were all negative. In 44 isolates Klebsiella pneumoniae, there are 4 isolates, whose DHA types were positive, and ATC-1 types were all negative. Conclusions Plasmid-mediated AmpC lactamase gene can be transferred to allogenic and xenogenic bacteria by conversion and conjugation. So they should be under careful surveillance.