生物
原肠化
胚胎
转录组
谱系(遗传)
注释
细胞生物学
细胞谱系
身份(音乐)
基因
计算生物学
遗传学
斑马鱼
进化生物学
Hox基因
中胚层
细胞分化
胚胎发生
基因表达
物理
声学
作者
Guangdun Peng,Shengbao Suo,Jun Chen,Weiyang Chen,Chang Liu,Fang Yu,Ran Wang,Shirui Chen,Na Sun,Guizhong Cui,Lu Song,Patrick P.L. Tam,Jing-Dong J. Han,Naihe Jing
标识
DOI:10.1016/j.devcel.2016.02.020
摘要
Gastrulation of the mouse embryo entails progressive restriction of lineage potency and the organization of the lineage progenitors into a body plan. Here we performed a high-resolution RNA sequencing analysis on single mid-gastrulation mouse embryos to collate a spatial transcriptome that correlated with the regionalization of cell fates in the embryo. 3D rendition of the quantitative data enabled the visualization of the spatial pattern of all expressing genes in the epiblast in a digital whole-mount in situ format. The dataset also identified genes that (1) are co-expressed in a specific cell population, (2) display similar global pattern of expression, (3) have lineage markers, (4) mark domains of transcriptional and signaling activity associated with cell fates, and (5) can be used as zip codes for mapping the position of single cells isolated from the mid-gastrula stage embryo and the embryo-derived stem cells to the equivalent epiblast cells for delineating their prospective cell fates.
科研通智能强力驱动
Strongly Powered by AbleSci AI