核酸
二茂铁
肽核酸
亚甲蓝
检出限
电极
电化学
生物传感器
化学
肽
氧化还原
纳米技术
生物化学
组合化学
催化作用
分子生物学
色谱法
材料科学
生物
有机化学
物理化学
光催化
作者
Pan Fu,Shu Xing,Mengjia Xu,Yang Zhao,Chao Zhao
标识
DOI:10.1016/j.snb.2019.127545
摘要
Herein we demonstrated a facile electrochemical method for simultaneous detection of miRNA21 and miRNA155 using a peptide nucleic acids (PNAs)-modified gold electrode coupled with the target-catalyzed hairpin assembly (CHA) strategy. In the presence of the target miRNA, the CHA was triggered selectively between two hairpins with one ferrocene (Fc) or methylene blue (MB) labelled. The resulting redox-active group modified CHA products (Fc-CHA21 or MB-CHA155) were then specifically captured by the PNA probes (PNA21 or PNA155) attached on the surface of a gold electrode, which bring the Fc and MB labels into close proximity to generate apparently enhanced electrochemical signals for sensitive and simultaneous detecting of low amount miRNA21 and miRNA155 in cancer cells. This assay was highly selective for discriminating miRNAs with similar sequences and has detection limits of 2.49 fM and 11.63 fM for miRNA21 and miRNA155, respectively. The feasibility of the method for sensitive determination of miRNA21 and miRNA155 from human cancer cells was also demonstrated. This method thus has great potential to be applied for simultaneous detecting of a variety of miRNA biomarkers for clinic applications due to its simple, sensitive and accurate features.
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