Scarcity of non-renewable energy sources has paved need for sustainable and renewable biofuels from biomass. Of all natural resources used for production of biofuel, lignocellulosic biomass (producing second generation biofuel) are most attractive as they are highly abundant and easily available throughout the year. Various measures for maximal and efficient utilization of these lignocellulosic biomass for production of biofuels (bioethanol, biodiesel and biogas) have been taken including pretreatment, saccharification and fermentation process.