Neobavaisoflavone Demonstrates Valid Anti-tumor Effects in Non-Small- Cell Lung Cancer by Inhibiting STAT3

Aim and Objective : Lung cancer is the most commonly occurring cancer, which contributes to the majority of death caused by cancer, where non-small-cell lung cancer (NSCLC) accounts for approximately 85% of lung cancer. To treat NSCLC, STAT3 has been identified as a target with therapeutic potential. The neobavaisoflavone (NBIF) is one of the flavonoids of traditional Chinese medicine Psoralea corylifolial. Materials and Methods: Human NSCLC cell lines, PC-9, H460 and A549, were applied to determine NBIF’s antiproliferative effects through cell viability and colony formation detection. The effect of NBIF on cell apoptosis was determined through Flow cytometry-based assay. Western blotting was used in this study to confirm the levels of P-STAT3 and Bcl-2 and Bax which are apoptotic proteins. Results: It was observed that NBIF could decrease the cell viability and migration and induce apoptosis in human NSCLC cell lines dose-dependently. Levels of P-STAT3, as well as the downstream signals of STAT3 pathway, were downregulated, suggesting that the tumor-suppression effects of NBIF might be related to the inhibition of STAT3 signaling. Furthermore, NBIF could contribute to the upregulation of BAX and downregulation of BCL2. Conclusion: NBIF might perform the anti-NSCLC efficacy as a result of the inhibition on STAT3 pathway. Besides, our work suggests that NBIF could provide therapeutic alternatives for NSCLC.