黄曲霉毒素
生物
卵黄囊
胃肠道
蛋黄
斑马鱼
转录组
炎症
脂质代谢
胚胎
基因表达
基因
细胞生物学
免疫学
生物化学
生物技术
食品科学
作者
Bence Ivánovics,Gyöngyi Gazsi,Marta Reining,Izabella Roberta Berta,Szilárd Póliska,Márta Tóth,Apolka Domokos,Béla Nagy,Ádám Staszny,Mátyás Cserháti,Éva Csősz,Attila Bácsi,Zsolt Csenki-Bakos,András Ács,Béla Urbányi,Zsolt Czimmerer
标识
DOI:10.1016/j.jhazmat.2021.125788
摘要
Aflatoxin B1-contaminated feeds and foods induce various health problems in domesticated animals and humans, including tumor development and hepatotoxicity. Aflatoxin B1 also has embryotoxic effects in different livestock species and humans. However, it is difficult to distinguish between the indirect, maternally-mediated toxic effects and the direct embryotoxicity of aflatoxin B1 in mammals. In the present study, we investigated the aflatoxin B1-induced direct embryotoxic effects in a zebrafish embryo model system combining toxicological, transcriptomic, immunological, and biochemical approaches. Embryonic exposure to aflatoxin B1 induced significant changes at the transcriptome level resulting in elevated expression of inflammatory gene network and repression of lipid metabolism and gastrointestinal tract development-related gene sets. According to the gene expression changes, massive neutrophil granulocyte influx, elevated nitric oxide production, and yolk lipid accumulation were observed in the abdominal region of aflatoxin B1-exposed larvae. In parallel, aflatoxin B1-induced defective gastrointestinal tract development and reduced L-arginine level were found in our model system. Our results revealed the complex direct embryotoxic effects of aflatoxin B1, including inhibited lipid utilization, defective intestinal development, and inflammation.
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