脐静脉
VCAM-1
下调和上调
单核细胞
细胞粘附
内皮干细胞
ICAM-1
间质细胞
细胞生物学
细胞粘附分子
基因敲除
粘附
生物
人脐静脉内皮细胞
癌症研究
细胞
化学
免疫学
体外
细胞培养
生物化学
基因
有机化学
遗传学
作者
Yan-Rou Bei,Shun-Chi Zhang,Yu Song,Mao-Lin Tang,Kelan Zhang,Min Jiang,Run-Chao He,Shao-Guo Wu,Xuehui Li,Limei Wu,Xiaoyan Dai,Yan‐Wei Hu
标识
DOI:10.1038/s41401-022-00923-5
摘要
Atherosclerosis is a chronic inflammatory disease of arterial wall, and circulating monocyte adhesion to endothelial cells is a crucial step in the pathogenesis of atherosclerosis. Epithelial-stromal interaction 1 (EPSTI1) is a novel gene, which is dramatically induced by epithelial-stromal interaction in human breast cancer. EPSTI1 expression is not only restricted to the breast but also in other normal tissues. In this study we investigated the role of EPSTI1 in monocyte-endothelial cell adhesion and its expression pattern in atherosclerotic plaques. We showed that EPSTI1 was dramatically upregulated in human and mouse atherosclerotic plaques when compared with normal arteries. In addition, the expression of EPSTI1 in endothelial cells of human and mouse atherosclerotic plaques is significantly higher than that of the normal arteries. Furthermore, we demonstrated that EPSTI1 promoted human monocytic THP-1 cell adhesion to human umbilical vein endothelial cells (HUVECs) via upregulating VCAM-1 and ICAM-1 expression in HUVECs. Treatment with LPS (100, 500, 1000 ng/mL) induced EPSTI1 expression in HUVECs at both mRNA and protein levels in a dose- and time-dependent manner. Knockdown of EPSTI1 significantly inhibited LPS-induced monocyte-endothelial cell adhesion via downregulation of VCAM-1 and ICAM-1. Moreover, we revealed that LPS induced EPSTI1 expression through p65 nuclear translocation. Thus, we conclude that EPSTI1 promotes THP-1 cell adhesion to endothelial cells by upregulating VCAM-1 and ICAM-1 expression, implying its potential role in the development of atherosclerosis.
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