动物科学
生物
基础(医学)
体内
金霉素
体重增加
沙门氏菌
空肠
作者
Fang Peng,Jinhui Yi,Jian Xiao,Junlie Chen,Haihan Zhang,Xi He,Zehe Song
摘要
Abstract This study was aimed to investigate the effects of dietary arctiin (ARC) supplementation (100, 200 and 400 mg/kg) on the growth performance and immune response of broilers after a Salmonella pullorum (S. pullorum) challenge, and we conducted in vitro anti-bacterial test to explore the bacteriostatic mechanism of ARC. The in vivo trial was randomly assigned to 6 groups: non-infected control group (NC) and positive control group (PC) received a basal diet; TET group, received a basal diet supplemented with 100 mg/kg chlortetracycline; ARC100, ARC200 and ARC400 groups received a basal diet containing 100, 200, 400 mg/kg ARC, respectively. From d 14 to d 16, all birds (except NC group) were infected with 1 mL (1×10 8 CFU/mL) fresh S. pullorum culture by oral gavage per day. In vivo results showed that dietary supplementation of 200 mg/kg ARC significantly increased average daily gain (P<0.05) and decreased feed-to-gain ratio of broilers versus the PC group during d 15-28 after challenged with S. pullorum (P<0.05). The jejunal crypt depth (CD) were decreased by supplementing 100 or 200 mg/kg ARC in diets compared with PC birds at d 19 (P<0.05). The jejunal villi height (VH) were increased by supplementing 100, 200 or 400 mg/kg ARC in diets compared with PC birds at d 28 (P<0.05). Besides, dietary supplementation of 200 mg/kg ARC increased the jejunal VH to CD ratio (VH:CD) than PC group both at d 19 and d 28 (P<0.05). Notably, the broilers had lower serum lipopolysaccharide (LPS) and diamine oxidase (DAO) levels in the ARC100 and ARC200 groups at d 28 than those in the PC group (P<0.05). Furthermore, in comparison to PC birds, the birds in ARC groups (100, 200, 400 mg/kg) had higher serum contents of IgM and IL-10, and the birds in ARC200 group had higher serum contents of IgA at d 19 (P<0.05). At d 28, the birds in ARC groups (100, 200, 400 mg/kg) had lower serum contents of IL-8, and the birds in ARC200 group had lower serum contents of IFN-γ compared to the PC birds (P<0.05). The in vitro experiment showed that ARC significantly inhibited the biofilm formation and adhesion of S. pullorum (P<0.05). Metabonomics analysis revealed that ARC can restrain the formation of the biofilm by affecting a variety of metabolic pathways of S. pullorum. Therefore, dietary supplementation of 200 mg/kg ARC might be a potentially way to substitute antibiotics to control S. pullorum infection in broilers.
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