化学
分子印迹聚合物
聚丙烯酰胺
聚合
检出限
选择性
电化学
单体
聚合物
膜
基质(化学分析)
电极
生物传感器
线性范围
化学工程
电化学气体传感器
色谱法
核化学
高分子化学
有机化学
生物化学
催化作用
物理化学
工程类
作者
Tianrun Zhang,Xiuwei Xuan,Mingji Li,Cuiping Li,Penghai Li,Hongji Li
标识
DOI:10.1016/j.aca.2022.339689
摘要
Molecularly imprinted polymer (MIP) membranes prepared in situ present several advantages: they maintain the original morphology, adhere strongly to the collector, and exhibit a controllable structure. In this study, a Ni-polyacrylamide (PAM)-MIP matrix was fabricated in situ on glassy carbon via the one-step electro-polymerization of AM monomers in the presence of Ni and template molecules. Ni2+ ions were introduced as oxidants to promote AM polymerization and bulking agents to fabricate a three-dimensional porous PAM-MIP matrix. The Ni-PAM-based MIP sensor exhibited a quantitative dual response toward dopamine (DA) and adenine (Ade) in the pH range of 5.0-9.0. The linear concentration range changed depending on the pH environment, and the concentrations of DA and Ade ranged from 0.6 to 200 μM and from 0.4 to 300 μM, respectively. The ranges of detection limits (S/N = 3) were 0.12-0.37 μM for DA and 0.15-0.36 μM for Ade. In addition, the dual-MIP sensor exhibited high reliability in the detection of DA and Ade in human serum owing to its excellent anti-interference ability and long-term stability. The technique developed in this study is expected to facilitate the construction of multi-target response electrochemical biosensors and the reliable determination of small molecules with high selectivity and stability.
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