Multiple pathways for the anaerobic biodegradation of microcystin-LR in the enriched microbial communities from Lake Taihu

Anaerobic biodegradation is a non-negligible elimination approach for microcystin (MC) pollution and exhibits important bioremediation potential for environmental problems. However, the specific anaerobic MC-degrading mechanism remains unclear and few functional bacteria have been found. In this study, three microbial communities of sludges from different locations in Lake Taihu were collected and further enriched by microcystin-LR (MC-LR) under anaerobic conditions. MC-LR (1 mg/L) could be completely degraded by these enriched microbial communities under anaerobic conditions, but their degradation rates were significantly different. In addition, two different ring-opening sites of MC-LR in Ala-Leu and Arg-Adda were observed, and three new anaerobic degradation products were first identified, including two hexapeptides (MeAsp-Arg-Adda-Glu-Mdha-Ala and Adda-Glu-Mdha-Ala-Leu-MeAsp) and one end-product pentapeptide (Glu-Mdha-Ala-Leu-MeAsp). Based on the chemical structures and temporal trends of all detected degradation products, two novel anaerobic biodegradation pathways of MC-LR were proposed. Moreover, the MC-degrading genes mlrABC were not detected among all microbial communities, which suggested that some new MC-degrading mechanisms might exist under anaerobic conditions. Finally, through the comparison of microbial community structure, Gemmatimonas and Smithella were deduced as possible anaerobic MC-degrading bacteria. These findings strongly indicate that anaerobic biodegradation is an important method of self-repair in the natural environment and provides a potential removal strategy for MC pollution.