Objective To investigate the current status of Clonorchis sinensis infection in Jiangmen, Xinhui City. Methods The specific antibody of Clonorchis sinensis in serum was tested by enzyme-linked immunosorbent assay (ELISA), and Clonorchis sinensis eggs in feces were detected by modified Kato-Katz method when the serum test was positive. The metacercaria of Clonorchis sinensis in fresh water fish were detected by digestion assay. Questionnaire was designed to collect the life style information about eating habits, etc. By using a case-control method, the risk factors of Clonorchis sinensis infection were also analyzed. Results 1 720 subjects were studied, and 18.14% of them were infected. The infection rate was significantly higher in male than in female. The metacercaria of Clonorchis sinensis was found in all 6 species of fresh water fish. Finally, eating behaviors, such as eating out and predilection for eating not-fully boiled fish, were the most significant risk factors. Conclusion The infection rate of Clonorchis sinensis in Xinhui was relatively higher,and it has been a severe public health problem. The comprehensive control measures should be actively conducted.