Effect of gallic acid on liver oxidative stress markers in renal ischemia-reperfusion injury in rats

Introduction: Renal ischemia-reperfusion (RIR) is one of the reasons of acute renal failure (ARF). Furthermore, RIR induces oxidative stress in the liver. Objectives: Considering the role of gallic acid (GA) as an antioxidant, we used GA to investigate its effect on liver oxidative stress markers in RIR injury in rats. Materials and Methods: Twenty-two adult male Sprague-Dawley rats were randomly divided into 3 groups; group I (control; non-ischemic animals, n = 8), group II (renal I/R injury, n = 7) and group III (renal I/R injury + GA 100 mg/kg i.p daily, n = 7). Pedicles were occluded for 45 minutes (ischemia) and subjected to 24 hours of reperfusion. Daily pretreatment began 15 days before the induction of RIR. Hepatic malondialdehyde (MDA) and glutathione (GSH) were determined, and catalase (CAT) and glutathione peroxidase (GPX) activities in the liver were assessed. Results: The level of MDA in the liver significantly decreased in treated RIR rats compared with the untreated group. The level of GSH in the liver increased in treated RIR rats compared with the untreated group. CAT and GPX activities in the liver were significantly less in the untreated group than the control one. GA significantly increased CAT and GPX activities in the liver of the treated group. Conclusion: Our study showed that the pretreatment with GA as an antioxidant agent has protective effects on liver oxidative stress markers in RIR injury in rats. Core tip Our study shows GA reduced the level of MDA and increased level of GSH and CAT and GPX activity in diabetic treated group. The authors hope, the results of the present study help to improve liver function and the complication in diabetic patients. Citation: Ahmadvand H, Babaeenezhad E, Hadipour-Moradi F, Cheraghi-Venool A. Effect of gallic acid on liver oxidative stress markers in renal ischemia-reperfusion injury in rats. Ann Res Antioxid. 2017;2(1):e03.