七鳃鳗
自噬
生物
先天免疫系统
斑马鱼
细胞生物学
免疫系统
自噬体
吞噬作用
吞噬体
基因
免疫学
遗传学
渔业
细胞凋亡
作者
Lingjie Luo,Juan Lü,Qin Wang,Shangwu Chen,Anlong Xu,Shaochun Yuan
标识
DOI:10.1016/j.fsi.2018.09.016
摘要
Autophagy is a homeostatic process which degrades cytoplasmic constituents to maintain the balance of organs when they were challenged with nutrient stress. It also participates in cancer, neurodegenerative disorders, aging and innate immune defense. In order to reveal how autophagy participates in innate immune response when invertebrates evolved into vertebrates. Firstly, we performed a systematic analysis of Atg genes and found that they are highly conserved among lancelet, lamprey and zebrafish. Then, we observed autophagosomes upon starvation by TEM in lancelet, lamprey and zebrafish and found that the morphology of autophagosome is similar to that was observed in yeast and mammals. In addition, rapamycin can induce autophagy in lamprey leukocytes and the deficiency of human Beclin1 protein can be rescued by lancelet and lamprey Beclin1 proteins. When lamprey leukocytes were treated with polyI:C and LPS, autophagy was induced. Moreover, when lamprey leukocytes were challenged with live E. coli, phagocytosis along with autophagy was triggered to degrade pathogenic bacteria. In all, our study here indicated that autophagy is highly conserved during evolution and plays a key role in innate defense when invertebrates evolved into vertebrates.
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