FGF mimetic peptide-modified electrospun nanocomposite fibrous membranes for accelerating infectious diabetic wound healing by synergistic antibacterial and pro-angiogenesis effects

Diabetic wound treatment remains a severe threat to public health. Biomimetic nanocomposite scaffolds have shown great potential in anti-infection, but the challenges associated with insufficient angiogenesis remain. Herein, an efficient nanocomposite membrane combining basic fibroblast growth factor (FGF2) mimetic peptides and copper/catechol-derived resin nanoparticles (CuCFR NPs) loaded poly(L-lactide-co-ε-caprolactone) (PLCL) electrospun fibrous membrane was developed for accelerating infectious diabetic wound healing by synergistic antibacterial and pro-angiogenesis effects. FGF2 mimetic peptides were chemically grafted onto the surface of the membrane to impart efficient cell viability while maintaining the porous ultrafine-fiber morphology, large tensile strength of 6.2 MPa and elongation of 317 %. The liberation of FGF2 mimetic peptides from the membrane effectively promoted both fibroblast and endothelial cell proliferation, migration, and enhanced tube formation in vitro. Importantly, owing to the unique structure of the CuCFR NPs, the membrane sustainedly released Cu2+ for 14 days, which effectively inhibited S. aureus (ca. 98 %) and modulated endothelial cell viability. Moreover, the membrane in vivo significantly reduced bacterial infection and promoted re-epithelialization, collagen deposition and angiogenesis in an infectious diabetic rat model. The peptide-modified nanocomposite membrane accelerates infectious diabetic wound healing and provides a new therapeutic perspective for the treatment of diabetic wounds.