烟酰胺磷酸核糖转移酶
化学
生物素化
肌发生
NAD+激酶
重组DNA
污渍
激活剂(遗传学)
甘油醛3-磷酸脱氢酶
生物化学
酶
分子生物学
受体
脱氢酶
体外
生物
基因
作者
Peng Lyu,Shengrong Li,Ying Han,Shengnan Shen,Zhaoyong Feng,Piliang Hao,Zhengqiu Li,Ligen Lin
标识
DOI:10.1016/j.bioorg.2023.106435
摘要
Herein, we synthesized an affinity-based probe of myricanol (pMY) with a photo-affinity cross-linker to initiate a bioconjugation reaction, which was applied for target identification in live C2C12 myotubes. Pull-down of biotinylated pMY coupled with mass spectroscopy and Western blotting revealed that pMY can bind with nicotinamide phosphoribosyltransferase (Nampt), a rate-limiting enzyme in the nicotinamide adenine dinucleotide salvage pathway. Cellular thermal shift assay, drug affinity responsive target stability assay and recombinant protein labeling further validated the direct interaction between myricanol and Nampt. Myricanol did not affect the protein expression of Nampt, but enhanced its activity. Knock-down of Nampt totally abolished the promoting effect of myricanol on insulin-stimulated glucose uptake in C2C12 myotubes. Taken together, myricanol sensitizes insulin action in myotubes through binding with and activating Nampt.
科研通智能强力驱动
Strongly Powered by AbleSci AI