表面等离子共振
费斯特共振能量转移
共振(粒子物理)
荧光团
膜
等离子体子
化学
纳米技术
化学物理
材料科学
物理
原子物理学
荧光
纳米颗粒
光学
光电子学
生物化学
作者
Qichun Zhang,Xuan Fang,Wenwen Huang,Li Qi,Hao Jiang,Chao Wang,Honglin Liu
出处
期刊:Nano Letters
[American Chemical Society]
日期:2023-06-30
卷期号:23 (16): 7750-7757
标识
DOI:10.1021/acs.nanolett.3c01629
摘要
Developing novel strategies to measure nanoscale distance and molecular interaction on a living cell membrane is of great significance but challenging. Here we develop a model of a linker-free plasmon resonance energy transfer, termed “PRET nanoruler”, which is composed of a single-sized nanogold-antibody conjugates donor (G26@antiCD71) and a fluorophore-labeled XQ-2d aptamer receptor (XQ-2d-Cy3), that produces a separation distance (r) dependent energy transfer (ηPRET). Both the theoretical finite element simulation and experiments evidence the observable PRET between single G26NPs and XQ-2d-Cy3. Regardless of the size of ηPRET, we could confirm r is less than 5 nm, the separation of two binding sites is in the range of 13.0–18.0 nm. There is a competitive binding of Tf and XQ-2d-Cy3 on CD71 receptors. PRET nanoruler realizes the estimation of the nanoscale separation distance, and determines the molecular interaction and competitive binding. It is an alternative tool for observing nanoscale single molecular events in the future.
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