Introduction of a multilayered fluorescent nanofilm into lateral flow immunoassay for ultrasensitive detection of Salmonella typhimurium in food samples

in 14 min with detection limits of as low as 9 cells/mL. Moreover, compared with traditional LFIA techniques for bacteria detection, the proposed assay exhibited excellent stability and accuracy in real food samples and enormously improved sensitivity (2-3 orders of magnitude), demonstrating its great potential in the field of rapid diagnosis.