Devitrite (Na2Ca3Si6O16) phase dominated nanostructured 45S5 bioactive glass: exploring its structural and biological properties

Abstract This research study is primarily centred around calcination temperature and time influence on phase formation in bioactive glasses (BGs). In the present study, BG with a nominal composition of 45S5 was synthesized through the sol–gel process. The developed BGs then underwent heat treatment for various sintering durations and temperatures. X-ray diffraction (XRD) patterns of the BGs reveals that the sintering process led to the crystallization of both devitrite (Na 2 Ca 3 Si 6 O 16 ) and combeite (Na 2 Ca 2 Si 3 O 9 ) phases. The field emission scanning electron microscopy study divulges morphological alterations, from sheet-like to rod-like structures to eventually transforming into spherical and sheet-like structures. The surface area and Type-IV mesoporous porosity were validated through Brunauer Emmett Teller analysis, highlighting a notable increase in pore volume and mechanical strength at a lower sintering temperature. In vitro apatite formation was carried out in Hank’s balance salt in order to evaluate the bioactivity of the glass. After 7 d of immersion in simulated body fluid (SBF), XRD patterns and scanning electron microscopy micrographs results showed that formation of hydroxyapatite layer on the surface of the BGs. The BG compatibility with erythrocytes (red blood cells) was also studied, and the results revealed that there was only a low 2% lysis, showing good hemocompatibility. The drug loading and release behaviour of the BGs was studied in the in vitro analysis. The findings showed a high drug encapsulation effectiveness of up to 90% and continuous drug release from the BGs for 24 h. The materials biocompatibility was unambiguously confirmed by cytocompatibility and proliferation studies. This study provides compelling evidence for the exceptional efficacy and promise of the distinct 45S5 BGs in advancing the field of regenerative medicine.